Unit 6: High-Performance Liquid Chromatography (HPLC)
This unit provides a comprehensive overview of High-Performance Liquid Chromatography (HPLC), covering its principle, system components, stationary and mobile phases, detectors, light sources, and performance verification standards. It explains the importance of HPLC in pharmaceutical analysis for assay, impurity profiling, and stability studies, aligned with ICH and USP guidelines.
HPLC separates components based on differential partitioning between a liquid mobile phase and a solid stationary phase under high pressure. Reverse-phase HPLC (RP-HPLC) is most common, using non-polar stationary phases and polar mobile phases.
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